I would like to put this little piece of code out there for as my title says: Feedback, Suggestions, Improvement Ideas if anyone is willing to look my code over! Thanks!

Code:
Option Strict On
Option Explicit On
Imports System.IO
Public Class Main

Public Class QuestionWithAnswers
        Public Property ItemNumber As Integer
        Public Property Question As String
        Public Property Answers As New List(Of Answer)
        Public Property CorrectAnswer As String
        Public Class Answer
            Public AnswerText As String
            Public AnswerChecked As Boolean
        End Class
    End Class

    Private AllQuestions As New List(Of QuestionWithAnswers)
    Private qIndex As Integer = 0
    Private updatingQuestion As Boolean
    Private curQandA As QuestionWithAnswers
    Private _myIndex As Integer = 1

    Private Sub BtnNext_Click(sender As Object, e As EventArgs) Handles btnNext.Click
        If AllQuestions.Count = 0 Then Exit Sub
        curQandA = AllQuestions.ElementAt(qIndex)
        If qIndex < 9 Then              ' <-----'Is it safe to Increment (also: how to change the qIndex integer up or down with button control if you want more than 10 questions in a quiz?)
            qIndex += 1                ' < -----'it is, 'then set the current question here since we are using an index
            LoadQuestion()
            UpdateLabel()
            lblCorrectAnswers.ResetText()
            rb1.Checked = False
            rb2.Checked = False
            rb3.Checked = False
            rb4.Checked = False
        End If
    End Sub

    Private Sub LoadQuestion()
        If AllQuestions.Count = 0 Then Exit Sub
        updatingQuestion = True
        curQandA = AllQuestions.ElementAt(qIndex)
        lbQuestion.Text = curQandA.Question
        rb1.Text = curQandA.Answers.ElementAt(0).AnswerText
        rb2.Text = curQandA.Answers.ElementAt(1).AnswerText
        rb3.Text = curQandA.Answers.ElementAt(2).AnswerText
        rb4.Text = curQandA.Answers.ElementAt(3).AnswerText
        rb1.Checked = curQandA.Answers.ElementAt(0).AnswerChecked
        rb2.Checked = curQandA.Answers.ElementAt(1).AnswerChecked
        rb3.Checked = curQandA.Answers.ElementAt(2).AnswerChecked
        rb4.Checked = curQandA.Answers.ElementAt(3).AnswerChecked
        updatingQuestion = False
    End Sub

    Sub UpdateLabel()
        lblNumber.Text = (qIndex + 1).ToString
    End Sub

    Private Sub Rbs_CheckedChanged(sender As Object, e As EventArgs) Handles rb4.CheckedChanged, rb3.CheckedChanged, rb2.CheckedChanged, rb1.CheckedChanged
        If Not updatingQuestion Then                     'when we call LoadQuestion we don't want any of these events to trigger
            Dim rb = DirectCast(sender, RadioButton)
            Dim qandaAnswer = curQandA.Answers.Where(Function(a) a.AnswerText = rb.Text).FirstOrDefault
            If Not qandaAnswer Is Nothing Then qandaAnswer.AnswerChecked = True
            For Each ans In curQandA.Answers
                If ans Is qandaAnswer Then
                    ans.AnswerChecked = True
                Else
                    ans.AnswerChecked = False
                End If
            Next
        End If
    End Sub

    Private Sub BtnPrevious_Click(sender As Object, e As EventArgs) Handles btnPrevious.Click
        If AllQuestions.Count = 0 Then Exit Sub
        curQandA = AllQuestions.ElementAt(qIndex)
        If qIndex > 0 Then
            qIndex -= 1
            LoadQuestion()
            UpdateLabel()
            rb1.Checked = False
            rb2.Checked = False
            rb3.Checked = False
            rb4.Checked = False
            lblPerRight.ResetText()
        End If
    End Sub

    Private Sub loadme(filename As String)
    Timer1.Enabled = True
        Using sr As New StreamReader(filename)
            While Not sr.EndOfStream
                Dim data() As String = sr.ReadLine.Split(","c)
                Dim qAndA As New QuestionWithAnswers
                qAndA.ItemNumber = Integer.Parse(data(0))
                qAndA.Question = data(1)
                Dim answers() = data(2).Split("|"c)
                For Each answer In answers
                    qAndA.Answers.Add(New QuestionWithAnswers.Answer With {.AnswerText = answer, .AnswerChecked = False})
                Next
                qAndA.CorrectAnswer = data(3)
                AllQuestions.Add(qAndA)
                UpdateLabel()
            End While
        End Using
        'get the first question qIndex starts at 0 - first element
        curQandA = AllQuestions.ElementAt(qIndex)
        LoadQuestion()
    End Sub

    Private Sub BtnSubmit_Click(sender As Object, e As EventArgs) Handles btnSubmit.Click
        If AllQuestions.Count = 0 Then Exit Sub
        Dim correctAnswers As Integer = 0
        For Each question As QuestionWithAnswers In AllQuestions
            For Each answer As QuestionWithAnswers.Answer In question.Answers                              '<---------How to validate if wrong answer picked?
                If answer.AnswerChecked AndAlso answer.AnswerText = question.CorrectAnswer Then
                    correctAnswers += 1
                    Exit For
                End If
            Next
        Next
        ' MessageBox.Show(String.Format("You have correctly answered {0} out of {1} questions!", correctAnswers, AllQuestions.Count.ToString))                 '<-----better grading system
      ' lblCorrectAnswers.Text = String.Format("You have correctly answered {0} out of {1} questions!", correctAnswers, AllQuestions.Count.ToString)
        lblPerRight.Text = String.Format("{0}% Correct!", correctAnswers * 10)
    End Sub
    Private Sub BtnTryAgain_Click(sender As Object, e As EventArgs) Handles btnTryAgain.Click
        rb1.Checked = False
        rb2.Checked = False
        rb3.Checked = False
        rb4.Checked = False
        For Each question As QuestionWithAnswers In AllQuestions
            For Each answer As QuestionWithAnswers.Answer In question.Answers
                answer.AnswerChecked = False
            Next
        Next
        qIndex = 0
        LoadQuestion()
        lblPerRight.ResetText()
        lblNumber.Text = "1"
        lblCorrectAnswers.ResetText()
    End Sub

    'Private Sub QuizItem_Click(ByVal sender As Object, ByVal e As System.EventArgs)

        'cast the "sender" "Leviticus (1).mp3"to a ToolStripMenuItem
        'Dim tsmi As ToolStripMenuItem = DirectCast(sender, ToolStripMenuItem)
       ' loadme(DirectCast(sender, ToolStripMenuItem).Tag.ToString)
        'convert the Tag (Object) back to a string to get the full file path name from the ToolStripMenuItem that was clicked
        'Dim FileToLoad As String = tsmi.Tag.ToString
        'just to show you the full file path and name from the ToolStripMenuItem Tag
        'AllQuestions.Clear()
       ' loadme(FileToLoad)
   ' End Sub
End Class
I put some questions in the comments of the code also.
Thanks for any input, its a project for my church quizzing team!