I would go into the BoundField properties for the GridView and then set the Width under the ItemStyle section.
If you have to do it programmatically you could always do something like
VB.NET:
Protected Sub GridView1_Init(ByVal sender As Object, ByVal e As System.EventArgs) _
Handles GridView1.Init
GridView1.Columns.Item(1).ItemStyle.Width = 250
End Sub
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